Dans l’Amphi du PBS

• Jeudi 24 mars, 11h00, Pierre BON (Laboratoire Photonique, Numerique et Nanosciences LP2N

CNRS, Institut d’Optique, Bordeaux, France)

« Imagerie quantitative de phase en microscopie optique pour les études biologiques et super-résolution » Séminaire technologique

(Invité par Anne CANTEREAU et ImageUP)

Quantitative phase imaging (QPI) is a powerful optical method to enhance the knowledge about a micro/nanoscopic samples. It relies on the capability to measure not only the intensity of the light but also its phase. This provides several types of information depending on the illumination scheme or imaging mode.

QPI was initially developed to image microscopic structures inside semi-transparent samples (ex. nucleus), without labelling/staining. I will show recent breakthrough using our QPI technique based on quadriwave lateral shearing interferometry [1] coupled with the native halogen trans-illumination of the microscope. It allows label-free cytoskeleton and organelle trafficking imaging at up to 50 Hz and for any duration [2].

I will also show how QPI can give a precise measurement of the dry mass inside living cells [3] and how it can be related to the cell cycle state.

I will finally present the capability to retrieve the 3D spatial distribution of particles (such as gold nanobeads) with a sub-nanometric localization by the simultaneous use of intensity and phase imaging. I will show the use of this method to stabilize the drift of a super-resolution microscope [4].

 

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[1] Bon, Maucort, Wattellier, and Monneret, « Quadriwave lateral shearing interferometry for quantitative phase microscopy of living cells, » Opt. Express 17, 13080-13094 (2009)

[2] Bon, Lécart, Fort, Lévêque-Fort, “Fast Label-Free Cytoskeletal Network Imaging in Living Mammalian Cells”, Biophysical J., 106, 1588 – 1595 (2014)

[3] Aknoun, Savatier, Bon, Galland, Abdeladim, Wattellier, and Monneret, « Living cell dry mass measurement

using quantitative phase imaging with quadriwave lateral shearing interferometry: an accuracy and sensitivity discussion, » J. of Biomedical Optics 20(12), 126009 (2015)

[4] Bon, Bourg, Lécart, Monneret, Fort, Wenger, Lévêque-Fort, “Three-dimensional nanometre localization of nanoparticles to enhance super-resolution microscopy”, Nat. Comm.,6 , 7764 (2015)